On Twitter, Mr Trump said the United States received “no appreciation or respect” in return for its aid.
He also said his controversial recognition of the contested city of Jerusalem as Israel’s capital took the hugely divisive issue “off the table” for new peace talks.
Palestinians had said the move showed the US could not be a neutral broker.
Soon after Washington’s decision was announced in December, Palestinian President Mahmoud Abbas said he would not accept any US peace plan for the Middle East.
The decision on Jerusalem was also overwhelmingly condemned at the United Nations, where 128 countries voted against Mr Trump’s fulfilment of a campaign promise.
The US President was tweeting a follow-up to earlier comments about aid payments to Pakistan, in which he said the US has received only “lies and deceit” in exchange for billions of dollars in aid.
It’s not only Pakistan that we pay billions of dollars to for nothing, but also many other countries, and others. As an example, we pay the Palestinians HUNDRED OF MILLIONS OF DOLLARS a year and get no appreciation or respect. They don’t even want to negotiate a long overdue.
We have taken Jerusalem, the toughest part of the negotiation, off the table, but Israel, for that, would have had to pay more. But with the Palestinians no longer willing to talk peace, why should we make any of these massive future payments to them?